Method and element for albumin assay

Chemistry: analytical and immunological testing – Peptide – protein or amino acid – Glycoproteins

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422 56, G01N 2178, G01N 3368

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active

045686475

ABSTRACT:
Disclosed herein is an assay for determination of albumin in a liquid sample. This assay involves the use of a dye of the structure: ##STR1## wherein n is zero or a positive integer up to 3; R.sup.1, R.sup.2 and R.sup.3 are independently hydrogen, alkyl, cycloalkyl, aryl, alkoxy, aroxy, hydroxy, carboxy, alkoxycarbonyl, amino or a nonaromatic heterocyclic group; and Z and Z.sub.1 independently represent the carbon, nitrogen, oxygen or sulfur atoms needed to complete a substituted or unsubstituted 5- to 7-membered carbocyclic or heterocyclic ring. This dye has a high affinity for albumin over a wide pH range. When the dye is bound to albumin, a shift in the dye spectral absorption occurs. Analytical elements containing this dye and an assay method are disclosed herein.

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