Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1990-12-27
1993-02-02
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 71, 435 75, 435 79, 435 792, 435975, 422 56, 422 61, C12Q 168
Patent
active
051837358
ABSTRACT:
A diagnostic element for detecting the presence of anti-dsDNA antibodies in a sample. The diagnostic element includes a support (e.g., a microcell), a coating of mBSA on the support, dsDNA antigens immobilized on the coating, and a blocking layer for blocking immobilization sites other than ones occupied by dsDNA antigens. A testing kit is also provided which includes a diagnostic element, a sample diluent, an antibody solution, and a label identifying solution.
REFERENCES:
Rubin et al: An Improved ELISA . . . Antibodies J. Immunol. Methods 63; pp. 359-366 (1983).
Vogt et al., J. Immunol. Methods 101 pp. 43-50 (1987).
Journal of Immunological Methods, vol. 101 issued 1987, R. F. Vogt, Jr. et al., "Quantitative differences among various proteins as blocking agents for ELISA microtiter plates", 43-50.
Clinical and Experimental Rheumatology, vol. 5, issued 1987, A. G. Tzioufas, et al., "Enzyme immunoassays for the detection of IgG and IGM anti-ds DNA antibodies: clinical significance and specificity", 247-253.
Journal of immunological Methods, vol. 48, issued 1987, Valesini et al., "The Trypanosoma Lewisi Immunoflorescense Test: A New Simple Technique for Simultaneous Determination of Total Antinuclear Antibodies and the Detection of Antibodies to double-stranded DNA" 177-188.
Journal of immunological methods, vol. 85, Issued 1985, Kenna et al., "Methods for Reducing Non-specific Antibody Binding in Enzyme-linked Immunosorbent Assays" 409-419.
Lopez Luis R.
Sterhan Marcia
Nucker Christine M.
Reaads Medical Products, Inc.
Stucker Jeffrey
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