Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2004-12-15
2009-10-06
Lam, Ann Y (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
Reexamination Certificate
active
07598033
ABSTRACT:
A peptide or protein microassay method and apparatus in which a wide variety of chromogenic or fluorogenic peptide or protein substrates of interest are individually suspended or dissolved in a hydrophilic carrier, with aliquots of each substrate being deposited in an array or microarray of reaction loci, or “dots.” Each dot, therefore, provides an individual reaction vessel containing the peptide or protein of interest, to which a biological sample may be applied for assay purposes. The sample is applied to the array or microarray of dots by one of a variety of focused sample application techniques, including aerosolizing or misting of the sample, or target application of the sample, onto each dot without creating fluid channels between the dots which would cause cross-contamination. In additional aspects, the present invention provides methods of transferring samples from an electrophoretic gel to a target plate for subsequent MALDI MS analysis. Chemical reactions of interest can be run directly on the target plate, and the reaction products on the target are then prepared for MALDI MS analysis by drying and aerosol deposition of matrix material, without the need for salt removal and additional processing steps.
REFERENCES:
patent: 4046513 (1977-09-01), Johnson
patent: 4877745 (1989-10-01), Hayes et al.
patent: 5345079 (1994-09-01), French et al.
patent: 5674698 (1997-10-01), Zarling
patent: 5738728 (1998-04-01), Tisone
patent: 5808300 (1998-09-01), Caprioli
patent: 5888733 (1999-03-01), Hyldig-Nielsen et al.
patent: 5985551 (1999-11-01), Brennan
patent: 6027890 (2000-02-01), Ness et al.
patent: 6207031 (2001-03-01), Adourian et al.
patent: 6225047 (2001-05-01), Hutchens et al.
patent: 6225061 (2001-05-01), Becker et al.
patent: 6288390 (2001-09-01), Siuzdak et al.
patent: 6316266 (2001-11-01), Nelson
patent: 6358692 (2002-03-01), Jindal et al.
patent: 6432360 (2002-08-01), Church
patent: 6521325 (2003-02-01), Engle et al.
patent: 6569383 (2003-05-01), Nelson et al.
patent: 6573369 (2003-06-01), Henderson et al.
patent: 6710335 (2004-03-01), Ellson et al.
patent: 6737024 (2004-05-01), Eipel et al.
patent: 6766817 (2004-07-01), daSilva
patent: 7297501 (2007-11-01), Diamond et al.
patent: 7332286 (2008-02-01), Diamond
patent: 2002/0146745 (2002-10-01), Natan et al.
patent: 2002/0195558 (2002-12-01), Ellson et al.
patent: 19628928 (1998-01-01), None
patent: 2332273 (1999-06-01), None
patent: WO 94/27719 (1994-12-01), None
patent: WO 98/16830 (1998-04-01), None
patent: WO 98/41531 (1998-09-01), None
patent: WO 00/04389 (2000-01-01), None
patent: WO 00/04390 (2000-01-01), None
patent: WO 00/54046 (2000-09-01), None
patent: WO 00/56442 (2000-09-01), None
patent: WO 02/062821 (2002-08-01), None
patent: WO 2005/059552 (2005-06-01), None
Miliotis et al., Protein identification platform utilizing micro dispensing technology interfaced to matrix-assisted laser desorption ionization time-of-flight mass spectrometry, 2000, J. Chromatography 886(1-2):99-110.
Fountoulakis et al., Identification of proteins by matrix-assisted laser desorption ionization-mass spectrometry following in-gel digestion in low-salt, nonvolatile buffer and simplified peptide recovery. 1997, Analytical Biochemistry 250(2):153-156.
Katayama et al., Optimization of in-gel protein digestion system in combination with thin-gel separation and negative staining in 96-well plate format, 2003, Rapid. Commun. Mass Spectrom. 17:1071-1078.
Reyzer et al., Direct analysis of drug candidates in tissue by matrix-assisted laser desorption/ionization mass spectrometry. J Mass Spectrom. 2003; 38: 1081-1092.
Chaurand et al., Direct profiling and imaging of peptides and proteins from mammalian cells and tissue sections by mass spectrometry. Electrophoresis. 23: 3125-3135, 2002.
Hadd et al., Microchip Device for Performing Enzyme Assays, 1997, Anal. Chem. 69:3407-3412.
Cohen et al., A Microchip-Based Enzyme Assay for Protein Kinase A, 1999, Analytical Biochem. 273: 89-97.
Butenas et al., Evaluation of the Initiation Phase of Blood Coagulation Using Ultrasensitive Assays for Serine Proteases, 1997, J. Biol. Chem. 272(34):21527-21533.
Morita et al., New Fluorgenic Substrates for α-Thrombin, Factor Xa, Kallikreins, and Urokinase. 1977, J. Biochem. 82:1495-1498.
Talanian et al., Substrate Specificities of Caspase Family Proteases, 1997, J. Biol. Chem. 272(15):9677-9682.
Thornberry et al., A Combinatorial Approach Defines Specificities of members of the Caspase Family and Granzyme B, 1997, J. Biol. Chem. 272(29):17907-17911.
Garcia-Calvo, Inhibition of Human Caspases by peptide-based and macromolecular inhibitors, 1998, J. Biol. Chem. 273(49):32608-32613.
Backes, Synthesis of positional-scanning libraries of fluirogenic peptide substrates to define the extended substrate specificity of plasmin and thrombin, 2000, Nature Biotech. 18:187-193.
Harris, Rapid and general profiling of protease specificity by using combinatorial fluoregenic substrate libraries, 2000, PNAS 97(14):7754-7759.
Butenas et al., Analysis of Tissue Plasminogen Activator Specificity Using Peptidyl Fluoregenic Substrates, 1997, Biochem. 36:2123-2131.
Rand et al., Blood Clotting in Minimally Altered Whole Blood, 1996, Blood 88(9):3432-3445.
Pryzdial et al., Plasmin Converts X from Coagulationzymogen to Fibrinolysis Cofactor, 1999, J. Biol. Chem. 274(13):8500-8505.
Newman et al., Ink-Jet Printing for the Fabrication of Amperometric Glucose Biosensors. 1992, Analytica Chimica Acta, Elsevier Sci. Pub., Amsterdam, 262:13-17.
Lemmo et al., Characterization of an Inkjet Chemical Microdispenser for Combinatorial Library Synthesis, 1997, Analytical Chemistry 69(4):543-551.
MacBeath et al., Printing Proteins as Microarrays for High-Throughput Function Determination, 2000, Science 289:1760-1763.
Zhu et al., Protein arrays and microarrays, 2001, Current Opinion in Chemical Biology, 5:40-45.
Adam et al., Profiling the specific reactivity of the proteome with non-directed activity-based probes, 2001, Chemistry & Biology 8:81-95.
Diamond Scott L.
Gosalia Dhaval
Lam Ann Y
Pepper Hamilton LLP
University of Pennsylvania
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