Method and devices for delivery of substrate for the detection o

Details Method and devices for delivery of substrate for the detection o Method and devices for delivery of substrate for the detection o

1992-09-15

1995-01-17

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

435 4, 435 71, 435 79, 435 14, 435 18, 435 19, 435 791, 435 21, 435 25, 435 28, 436169, 436170, 436807, C12P 100, C12Q 134, C12Q 126, G01N 2177

Patent

active

053825160

ABSTRACT:
The invention provides a method for supplying an enzyme substrate to a membrane-based, enzyme-linked reaction, comprising providing an open pore, high liquid retention capacity material impregnated with a predetermined amount of a substrate for the enzyme; and contacting the material with a membrane containing the enzyme-linked reaction under conditions which permit diffusion of the enzyme substrate to sites on the membrane containing the enzyme linked reaction. The invention further provides devices for membrane-based enzyme-linked reactions comprising a porous material impregnated with a predetermined amount of a substrate for the enzyme, wherein the porous material is paper, glass fiber paper, stainless steel mesh, woven and nonwoven nylon, polyester, polyethylene, polypropylene, or fluorocarbon polymers having a porosity ranging from about 5 microns to 5000 microns; and a microporous membrane of cellulose or a cellulose derivative, nylon, polysulfone, polypropylene, PTFE, or PVDF having a porosity ranging from about 0.05 microns to about 12 microns.

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