Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1993-12-09
1997-11-11
Arthur, Lisa B.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 2, 435 4, 435 6, 435 71, 210789, 210782, C12Q 170, C12Q 100, C12Q 168, G01N 3353, B01D 3327, B01D 2126
Patent
active
056862385
ABSTRACT:
A method and apparatus for creating a leukocyte rich sample from a mixed population of blood cells. The method comprises the steps of: separating an amount of blood into layers that include a buffy coat layer having a mixed population of blood cells including plasma and red blood cells; providing a chamber having an opening for receiving a fluid; causing the buffy coat layer to enter the chamber; centrifuging the chamber to separate the buffy coat layer into at least a plasma containing layer, a leukocyte containing layer, and a red blood cell containing layer; and separating the leukocyte containing layer from the other layers in the chamber.
REFERENCES:
patent: 4223672 (1980-09-01), Terman et al.
patent: 5071570 (1991-12-01), Shiraki et al.
patent: 5089146 (1992-02-01), Carmen et al.
patent: 5102407 (1992-04-01), Carmen et al.
patent: 5154716 (1992-10-01), Bauman et al.
patent: 5334499 (1994-08-01), Burdick et al.
patent: 5342753 (1994-08-01), Smith, Jr.
patent: 5399268 (1995-03-01), Pall et al.
Arne B.o slashed.yum, "Separation of Lymphocytes, Granulocytes, and Monocytes from Human Blood Using Iodinated Density Gradient Media" Methods in Enzymology, V. 108, 1984, pp. 88-102.
Bhupat D. Rawal, et al., "Evaluation of Leukocyte Removal Filters Modelled by Use of HIV-Infected Cells and DNA Amplification", Blood, vol. 76, No. 10 (Nov. 15, 1990) pp. 2159-2161.
Don W. Sutton, et al., "Cell Separation in the Buffy Coat," Biorheology: 25; 1988, pp. 663-673.
Barbara, John A.J. and Garson, Jeremy A.: "Polymerase Chain Reaction and Transfusion Microbiology," Vox Sang 1993, pp. 73-81, vol. 64.
Bruisten, S.M. et al.: "Efficiency of White Cell Filtration and a Freeze-Thaw Procedure for Removal of HIV-infected Cells from Blood," Transfusion, vol. 30, No. 9, Nov.-Dec., 1990, pp. 833-837.
Frewin, D.B. et al.: "A Comparative Study of the Effect of Three Methods of Leukocyte Removal on Plasma Histamine Levels in Stored Human Blood," Seminars in Hematology, vol. 28, No. 3, 1991, pp. 18-21.
Hogman, Claes F. et al.: "The Bottom and Top System: A New Technique for Blood Component Preparation and Storage," Vox Sang 1988, pp. 211-217, vol. 55.
Keller, George H. and Manak, Mark M.: "DNA Probes," Macmillan Publishers, Ltd., Great Britain, 1993, pp. 36-39; 385-403.
Perrow, Jenny G.: "The Preparation of Leucocyte Homogenates for Enzymatic Assays," New Zealand Medical Lab. Technology, Mar., 1977, pp. 6-9, vol. 25(4).
Howell, Clifford L. et al. "Comparison of Rates of Virus Isolation from Leukocyte Populations Separated from Blood by Conventional and Ficoll/Paque/Macrodex Methods", in Journal of Clinical Microbiology, Oct. 1979, pp. 533-537, vol. 10, No. 4.
Maniatis et al. (1982) Molecular Cloning: A Lab. Manual Cold Spring Harbor Lab, pp. 80-83.
Harlowe et al. (1988) Antibodies: A Lab Manual, Cold Spring Harbor Lab. pp. 584-589.
Bratten William
Chapman John
Martinson Jeffrey
Wang Li Ming
Arthur Lisa B.
Barrett Robert M.
Baxter International Inc.
Price Bradford R. L.
Serewicz Denise M.
LandOfFree
Method and device for testing blood units for viral contaminatio does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method and device for testing blood units for viral contaminatio, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method and device for testing blood units for viral contaminatio will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1227772