Liquid purification or separation – Processes – Separating
Patent
1987-04-27
1989-08-01
Jones, W. Gary
Liquid purification or separation
Processes
Separating
210516, 422101, 422102, 436177, 494 16, 494 37, 494 38, B01D 2126
Patent
active
048531375
DESCRIPTION:
BRIEF SUMMARY
BACKGROUND OF THE INVENTION
This invention relates to a method of separating serum/plasma included in a certain volume of blood, said volume of blood being introduced into a test tube, in which a so-called separating body is also present, after which the test tube is centrifugalized, the blood being separated into a heavy phase containing red blood corpuscles and a light phase containing serum or plasma, the separating body being given such a specific gravity that upon centrifugation it enters a position in the boundary layer between the heavy and light phase. The invention also relates to a device for separation according to the above.
A plurality of various technical methods are known to provide separation of a sample of blood enclosed in a test tube to a heavy phase containing red blood corpuscles and a light phase containing serum.
A general method is arranging a silicon compound having a specific gravity between that of the heavy phase and the light phase on the bottom of a test tube. Upon centrifugation of the sample the silicon compound will form a barrier between the heavy phase and the light phase so that the light phase, i.e. the serum/ plasma can be removed from the test tube while the heavy phase, i.e. the red blood corpuscles, remains in the test tube. However, this technique does not provide any mechanical barrier between the two phases and, moreover, the accumulation of leukocytes and trombocytes in the boundary layer between the two phases is not taken charge of, meaning that the serum/plasma will be "contaminated" by these leukocytes and trombocytes.
It is also known in separation of the kind described above to use a quite mechanical separating body having such as specific gravity that it will adjust itself upon centrifugation of the test tube in the boundary layer between the heavy and the light phase.
The mechanical separating bodies have different types of seals bearing on the walls of the test tube. However, these seals cannot be made too efficient as the separating body must be able to slide within the test tube. Moreover, the accumulation of leukocytes and trombocytes in the boundary layer between the heavy and the light phase is not taken charge of but will "contaminate" the serum/plasma.
SUMMARY OF THE INVENTION
It is the object of this invention to show a method and a device of the kind mentioned above, where the separation is achieved by means of a separating body providing, on one hand, an adequate mechanical sealing between the two phases and, on the other hand, takes charge of the concentration of leukocytes and trombocytes taking place in a separation through centrifugation.
This object of the invention is realized by a mechod and a device to which have been given the characteristic features defined in the claims.
According to a preferred embodiment of the invention the separating body is arranged in a test tube open at both ends, said ends being sealed by means of elastic members and, as a result of this, vacuum prevailing in the test tube.
BRIEF DESCRIPTION OF THE DRAWING
An illustrative example of the invention will be described below with reference to the enclosed drawings, wherein
FIG. 1 shows a test tube in which a separating body according to the invention is placed and the test tube is in a position of sampling;
FIG. 2 shows the test tube according to FIG. 1 in a position for separation;
FIG. 3 shows the test tube according to FIG. 1 after separation;
and FIG. 4 shows an alternative embodiment of the separating body according to the invention.
DETAILED DESCRIPTION
The test tube 1 shown in FIGS. 1-3 is open at its two ends. One end (the upper one in FIG. 1) of the test tube 1 is sealed by means of a plug 2 of an elastic material, said plug 2 being of a type that can be penetrated a plurality of times by a cannula 3 without losing its sealing ability upon removal of the cannula 3.
At the other end of the test tube 1 (the lower one in FIG. 1) a relatively thin membrane 4 of an elastic material is arranged which is threaded onto said end of the test tube 1 to pr
REFERENCES:
patent: 3957654 (1976-05-01), Ayres
patent: 4055501 (1977-10-01), Cornell
patent: 4088582 (1978-05-01), Murty et al.
patent: 4190535 (1980-02-01), Luderer et al.
patent: 4417981 (1983-11-01), Nugent
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