Method and device for rapid detection of microorganisms by...

Details Method and device for rapid detection of microorganisms by... Method and device for rapid detection of microorganisms by...

2009-04-27

2011-11-29

Prouty, Rebecca (Department: 1653)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

C435S029000, C435S174000, C435S176000

Reexamination Certificate

active

08067154

ABSTRACT:
This invention describes a method of rapid detection of micro-colonies of microorganisms by changing their shape from a regular semi-sphere to a long and thin cylinder. Cells are trapped by filtration in long (diameter/length=1/10-1/100), cylindrical, parallel, micro-channels that are open from both sides, and attached to a filter from one side. A micro-channel plate houses a multiplicity of micro-channels (diameter of each channel=1-20 μm, and length 100-1000 μm). The micro-channel plate with cells trapped on the surface of the filter is attached to a nutrient media agar block. Cells produce micro-colonies of a long and thin shape according the shape of the micro-channel. The growth of microorganisms in the micro-channels permits a change in the number of cells to accomplish light absorbance. Fewer cells need a shorter time to reproduce. Thus detection and counting of cells can be accomplished in a rapid fashion.

REFERENCES:
patent: 2006/0240543 (2006-10-01), Folch et al.

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