Optics: measuring and testing – By dispersed light spectroscopy – With sample excitation
Reexamination Certificate
2006-03-07
2006-03-07
Lee, Andrew H. (Department: 2877)
Optics: measuring and testing
By dispersed light spectroscopy
With sample excitation
C359S022000
Reexamination Certificate
active
07009700
ABSTRACT:
A method and device for obtaining a sample with three-dimensional microscopy, in particular a thick biological sample and the fluorescence field emitted by the sample. One embodiment includes obtaining interferometric signals of a specimen, obtaining fluorescence signals emanating from the specimen, recording these signals, and processing these signals so as to reconstruct three-dimensional images of the specimen and of the field of fluorescence emitted by the specimen at a given time. Another embodiment includes a digital holography microscope, a fluorescence excitation source illuminating a specimen, where the microscope and the fluorescence excitation source cooperate to obtain interferometric signals of the specimen and obtain fluorescence signals emanating from the specimen, means for recording the interferometric signals and fluorescence signals, and means for processing the interferometric signals and the fluorescence signals so as to reconstruct three-dimensional images of the specimen and of the field of fluorescence emitted by the specimen at a given time.
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F. Dubois, L. Joannes, and J. Legros, “Improved three-dimensional imaging with a digital holography microscope with a source of partial spatial coherence,” Applied Optics vol. 38, No. 34 ,pp. 7085-7094 (1999).
Dubois et al., “Improved three-dimensional imaging with a digital holography microscope with a source of partial spatial coherence,”Applied Optics, vol. 38, No. 34, pp. 7085-7094, Dec. 1999.
Dubois Frank
Yourassowski Catherine
Knobbe Martens Olson & Bear LLP
Lee Andrew H.
Universite Libre De Bruxelles
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