Method and device for isoelectric focusing without carrier ampho

Details Method and device for isoelectric focusing without carrier ampho Method and device for isoelectric focusing without carrier ampho

1995-03-24

1998-06-02

Tsang, Cecilia J.

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

204451, 204601, 204610, C25B 900

Patent

active

057593701

ABSTRACT:
A device and method for isoelectric focusing of ampholytes in a buffer uses a cone-shaped capillary with a positive electrode connected at a narrow end and a negative electrode connected at the wide end of the capillary. The electrical potential across the buffer creates a temperature gradient which, in turn, creates a pH gradient. The electric current also creates an electric field gradient which focuses the ampholytes. Previous devices and methods use carrier ampholytes or use temperature baths to create a temperature gradient.

REFERENCES:
patent: 3664939 (1972-05-01), Luner et al.
patent: 4725343 (1988-02-01), Hjerten et al.
patent: 5110434 (1992-05-01), Zhu et al.
patent: 5324413 (1994-06-01), Gordon

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