Method and device for improved restriction fragment length polym

Details Method and device for improved restriction fragment length polym Method and device for improved restriction fragment length polym

1991-08-26

1994-06-28

Fleisher, Mindy B.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 2433, 935 77, 935 78, C12Q 168, C12P 1934, C07H 2104, C12N 1500

Patent

active

053246314

ABSTRACT:
Improvements in the efficiency and sensitivity of restriction fragment length polymorphism (RFLP) detection in target sequences are realized by developing primers that are specific for nucleic acid sequences, labelling those primers with distinguishable, non-radioactive labels, such as chromophores, and applying the primers in sets such that, after amplification of the target sequence, specific sequences, in particular those of RFLPs, can be identified by virtue of the probe(s) which hybridized.

REFERENCES:
patent: 4683194 (1987-07-01), Saiki et al.
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4729947 (1988-03-01), Midderdorf et al.
patent: 4795699 (1989-01-01), Tabor et al.
patent: 4965188 (1990-10-01), Mullis et al.
Hopman et al., Histochemistry, (1986), 85:1-4.

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