Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1992-08-04
1994-05-10
Parr, Margaret
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435287, 435291, 435299, 435301, 435310, 435810, 436501, 536 221, 536 231, 536 2431, 536 2432, 536 2433, 935 78, C12Q 168
Patent
active
053106504
ABSTRACT:
The present invention relates to methods and test strips for performing nucleic acid hybridizations on a porous chromatographic material having the capacity for rapid chromatographic solvent transport of non-immobilized reagents and reactive solution components by means of a selected chromatographic solvent. The strip includes a first end or contact site, at which chromatographic solvent transport begins, a second end, at which chromatographic solvent transport ends, and at least one zone positioned between the first and second ends. The zone is impregnated with a capture nucleotide sequence which is immobilized against solvent transport and which is capable of hybridization with a complementary nucleotide sequence or probe so as to render it immobilized in the capture zone. The strip may further include a probe zone between the first end and the capture zone, wherein a labeled probe is impregnated such that it is mobilized by the solvent and transported to the capture zone where hybridization can occur. Interfering sample components and unhybridized nucleic acids are cleared from the capture zone by chromatographic solvent transport to form an inherent wash.
REFERENCES:
patent: 4299916 (1981-11-01), Litman et al.
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4787963 (1988-11-01), MacConnell
patent: 4806311 (1989-02-01), Greenquist
patent: 4960691 (1990-10-01), Gordon et al.
Jeppessen (1971) Biochem., J., vol. 124, pp. 357-366.
Brownlee et al. (1969) Eur. J. of Biochem., vol. 11, pp. 395-399.
Domdey et al. (1979) Anal. Biochem., vol.98, pp. 346-352.
Gordon Julian
McMahon Michael E.
Abbott Laboratoires
Brainard Thomas D.
Marschel Ardin H.
Parr Margaret
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