Method and device for identifying substances capable of...

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...

Reexamination Certificate

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C435S377000, C435S440000, C435S455000

Reexamination Certificate

active

10501525

ABSTRACT:
The invention concerns a method for identifying compounds capable of modulating adipocyte differentiation, which consists in contacting a compound to be tested with genetically modified pre-adipocyte cells overexpressing the REV-ERB ALPHA receptor and measuring the adipocyte differentiation of said genetically modified cells as compared with the adipocyte differentiation of same said genetically modified pre-adipocyte cells in the absence of said compound to be tested.The invention also concerns genetically modified pre-adipocyte cells overexpressing the REV-ERB ALPHA receptor as well as the method for preparing said cells.

REFERENCES:
patent: WO 99/67637 (1999-12-01), None
patent: WO 02/058532 (2002-08-01), None
Austin et al, “Induction of the Nuclear Orphan Receptor RORγ during Adipocyte Differentiation of D1 and 3T3-L1 Cells”, Cell Growth and Differentiation, vol. 9, Mar. 1998, pp. 267-276.
Adelmant et al, “A functional Rev-erbα responsive element located in the human Rev-erbα promoter mediates a repressing activity”, Proceedings of the National Academy of Sciences, vol. 93, Apr. 1996, pp. 3553-3558.
Database Accession No. P20393, XP002226720, Feb. 1, 1991.
Chawla et al, “Induction of Rev-ErbAα, an Orphan Receptor Encoded on the Opposite Strand of a α-Thyroid Hormone Receptor Gene, during Adipocyte Differentiation”, The Journal of Biological Chemistry, vol. 268, No. 22, 1993, pp. 16265-16269.
Chawla et al, “Peroxisome proliferator and retinoid signaling pathways co-regulate preadipocyte phenotype and survival”, Proceedings of the National Academy of Sciences, vol. 91, No. 5, Mar. 1, 1994, pp. 1786-1790.
Gervois et al, “Fibrates Increase Human REV-ERBα Expression in Liver via a Novel Peroxisome Proliferator-Activated Receptor Response Element”, Molecular Endocrinology, vol. 13, Mar. 1999, pp. 400-409.
Fontaine et al, “The Orphan Nuclear Recdeptor Rev-Erbα Is a Peroxisome Proliferator-activated Receptor (PPAR) γ Target Gene and Promotes PPAR γ-induced Adipocyte Differentiation”, The Journal of Biological Chemistry, vol. 278, No. 39, Sep. 26, 2003, pp. 37672-37680.

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