Method and device for extraction and purification of DNA

Details Method and device for extraction and purification of DNA Method and device for extraction and purification of DNA

1997-11-13

1998-11-10

Redding, David A.

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

4352971, 4353041, 4353061, 210266, 210283, 210287, 210290, C12M 300

Patent

active

058343032

ABSTRACT:
A method for the extraction and purification of a plasmid DNA which comprises (1) collecting a culture medium containing a transformant into a first cartridge for the extraction and purification of the DNA, (2) subjecting said transformant to bacteriolysis and RNA degradation conditions, (3) recovering an extract containing solubilized DNA from said first cartridge, (4) transferring said recovered extract to a second cartridge for the extraction and purification of the DNA, and (5) absorbing, washing and eluting purified plasmid DNA from said second cartridge; and a device for the extraction and purification of a DNA which comprises a first cartridge for the extraction and purification of the DNA having at least a trap filter and a membrane filter, and a second cartridge for the extraction and purification of the DNA having at least a glass fiber filter, a glass powder layer and a membrane filter.

REFERENCES:
patent: 5329000 (1994-07-01), Woodard et al.
patent: 5330916 (1994-07-01), Williams et al.
patent: 5342931 (1994-08-01), Woodard et al.
patent: 5405951 (1995-04-01), Woodard
patent: 5438127 (1995-08-01), Woodard et al.
Preparation and Analytical Purification of DNA from Agarose, B. Vogelstein et al., Proc. Natl. Acad.Sci.USA, vol. 76, No. 2, pp. 615-619, Feb. 1979.
A Procedure For the Large-Scale Isolation of Highly Purified Plasmid DNA Using Alkaline Extraction and Binding to Glass Powder, M. A. Marko et al., Analytical Biochemistry, 121, 1982, pp. 382-387.

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