Chemistry: molecular biology and microbiology – Apparatus – Mutation or genetic engineering apparatus
Reexamination Certificate
2008-04-15
2008-04-15
Russel, Jeffrey Edwin (Department: 1654)
Chemistry: molecular biology and microbiology
Apparatus
Mutation or genetic engineering apparatus
C435S173600, C435S461000
Reexamination Certificate
active
10346755
ABSTRACT:
Described are methods for electrical treatment of biological cells, in particular using electrical field pulses, involving the steps: arrangement of the cells (1) on apertures (2) of a solid planar carrier element (3) which divides a measuring chamber (10) into two compartments (11, 12); and temporary formation of an electrical treatment field which permeates the cells, wherein an alternating-current impedance measurement takes place on the carrier element (3), and from the result of the alternating-current impedance measurement, a degree of coverage of the carrier element and/or healing of the cells after electrical treatment are/is acquired. Also described are devices for implementing the methods.
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Huang, Yong, et al.; “Microfabricated electroporation chip for single cell membrane permeabilization”, 2001 Elsevi Science B. V. , Sensor and Actuators A 89 (2001) 242-249.
Lucas Kurt
Sukhorukov Vladimir
Zimmermann Ulrich
Drinker Biddle & Reath
Eppendorf AG
Koons, Esq. Robert A.
Russel Jeffrey Edwin
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