Method and device for electroporation of biological cells

Details Method and device for electroporation of biological cells Method and device for electroporation of biological cells

2008-04-15

2008-04-15

Russel, Jeffrey Edwin (Department: 1654)

Chemistry: molecular biology and microbiology

Apparatus

Mutation or genetic engineering apparatus

C435S173600, C435S461000

Reexamination Certificate

active

10346755

ABSTRACT:
Described are methods for electrical treatment of biological cells, in particular using electrical field pulses, involving the steps: arrangement of the cells (1) on apertures (2) of a solid planar carrier element (3) which divides a measuring chamber (10) into two compartments (11, 12); and temporary formation of an electrical treatment field which permeates the cells, wherein an alternating-current impedance measurement takes place on the carrier element (3), and from the result of the alternating-current impedance measurement, a degree of coverage of the carrier element and/or healing of the cells after electrical treatment are/is acquired. Also described are devices for implementing the methods.

REFERENCES:
patent: 6300108 (2001-10-01), Rubinsky et al.
patent: 6927049 (2005-08-01), Rubinsky et al.
patent: 6936462 (2005-08-01), Owen et al.
patent: WO93/02178 (1993-02-01), None
patent: WO01/07583 (2001-02-01), None
patent: WO01/07584 (2001-02-01), None
patent: WO01/07585 (2001-02-01), None
Huang, Yong, et al.; “Microfabricated electroporation chip for single cell membrane permeabilization”, 2001 Elsevi Science B. V. , Sensor and Actuators A 89 (2001) 242-249.

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