Radiant energy – Luminophor irradiation – With ultraviolet source
Patent
1993-11-10
1996-06-18
Hannaher, Constantine
Radiant energy
Luminophor irradiation
With ultraviolet source
G01N 2164
Patent
active
055280463
ABSTRACT:
The present invention is directed to a device and method for determining the presence of fluorescent molecules, locations thereof, and number of fluorescent molecules in a local area irradiated with excitation light based on the number of fluorescence photons generated per unit period of time by the irradiation of the excitation light counted as a quantized fluorescence intensity of the fluorescence molecules and a distribution of appearance frequencies of the fluorescence photons formed on a two-dimensional image. In accordance with the present invention, a device is provided which comprises a light source for irradiating excitation light to a local area on a flat substrate with the adsorbed fluorescent molecules, and fluorescence detecting means for detecting fluorescence photons from the fluorescent molecules and for forming a distribution of appearance frequencies of the fluorescence photons per unit period of time on a two-dimensional image corresponding to the local area.
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Hamamatsu Photonics K.K.
Hannaher Constantine
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