Method and device for determining protein using carrier matrix c

Details Method and device for determining protein using carrier matrix c Method and device for determining protein using carrier matrix c

1990-05-29

1992-03-17

Hill, Jr., Robert J.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

422 56, 422 57, 427 2, 4274071, 428241, 428243, 428260, 428160, 428290, 4284231, 436 88, 436169, 436170, 435805, 435970, 521159, G01N 2178, G01N 3368

Patent

active

050968335

ABSTRACT:
A test device and method of determining the presence and concentration of proteins in a test sample are disclosed. The test device includes a test pad comprising a new and improved carrier matrix incorporating an indicator reagent composition capable of interacting with proteins to produce a detectable or measurable response. The new and improved carrier matrix of the test pad comprises a film, membrane or layer of a polymerized urethane-based compound, a water insoluble inorganic compound and an insoluble organic compound. The carrier matrix provides improved color resolution and increased sensitivity to proteins in dry phase test strip assays, thereby achieving an accurate and trustworthy protein assay of a liquid test sample, such as urine, having a protein concentration as low as about 5 mg/dL. Also disclosed is a method of making the test device.

REFERENCES:
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patent: 3438737 (1969-04-01), Atkinson et al.
patent: 3485587 (1969-12-01), Keston
patent: 4166093 (1979-08-01), Smith-Lewis et al.
patent: 4438067 (1984-03-01), Siddiqi
patent: 4466931 (1984-08-01), Tanny
patent: 4661526 (1987-04-01), Ford

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