Method and device for determining distribution of glycation...

Chemistry: analytical and immunological testing – Biological cellular material tested

Reexamination Certificate

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C435S325000

Reexamination Certificate

active

10903077

ABSTRACT:
A method for determining the distribution of glycation of erythrocytic membranes includes steps of bonding magnetic beads having a boric acid group with sugars on the surfaces of erythrocytic membranes, and moving the erythrocytes bonded with the boric acid-magnetic beads under an influence of an electromagnetic force. A device for determining the distribution of glycation of erythrocytic membranes includes a container as a cell for electromagnetic-phoresis, a cathode ray and an anode ray which are connected to a direct current power supply, and a magnetic sheet which is placed outside the bottom of the container. The container has an upper surface containing a cathode hole, an anode hole, a filling hole midway between the cathode hole and the anode hole for a specimen, and an excretory hole for releasing air bubbles. After physiological saline is poured into the container, the cathode ray and the anode ray are respectively inserted into the holes to be soaked in the saline. Subsequently, a specimen of erythrocytes, which are bonded with the boric acid-magnetic beads, is infused into the container through the filling hole.

REFERENCES:
patent: 4452773 (1984-06-01), Molday
patent: 5968820 (1999-10-01), Zborowski et al.
patent: 6162645 (2000-12-01), Lee et al.
patent: 08-122335 (1996-05-01), None
patent: 09-178719 (1997-07-01), None
Umudum, FZ et al. Erythrocyte membrane glycation and Na+-K+ levels in NIDDM. Journal of Diabetes and Its Complications. 2002. 16: 359-362.

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