Method and device for determination of parameters of individual

Optics: measuring and testing – For size of particles – By particle light scattering

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G01N 1502

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056508476

ABSTRACT:
A method and device for determination of absolute values of the size and the refractive index of individual microparticles. An optical scanning device for measurement of angular scattering pattern from individual microparticles is described. The light scatter is measured in different angles from moving particles inside the scanning optical system and the values and the angular locations of minima and maxima of the scattering pattern are registered. The size and the refractive index of individual microparticles are calculated from the registered minima and maxima using an empirical equation. An empirical equation has been derived from statistical analysis of angular scattering patterns. These patterns have been calculated on the basis of Mie scattering theory.

REFERENCES:
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P.J. Wyatt, "Discrimination of Phytoplankton via Light-Scattering Properties", 34 Limnology and Oceanography, 96-112 (1989).
Maltsev et al., "Estimation of morphological characteristics of single particles from light scattering data in flow cytometry," Russian Chem. Bull. 43, 1181-1190 (Jul. 1984) (with English language abstract).
Phillips et al., "Measurement of the Lorenz-Mie Scattering of a Single Particle: polystyrene latex", Colloid and Interface Science, 1970, 34, p. 159.
P.J. Wyatt, "Light Scattering in the Microbial World", Colloid and Interface Science, 1972, v. 39, pp. 479-491.
V.R. Stull, "Size Distribution of Bacterial Cells," J. Bacteriol., 1972, 109, pp. 1301-1303.
Wyatt et al., "Aerosol Particle Analyzer", Applied Optics, 1988 27 (2), pp. 217-221.

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