Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2004-04-29
2010-02-02
Aeder, Sean E (Department: 1642)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S007100
Reexamination Certificate
active
07655404
ABSTRACT:
A method for the detection and/or quantification of at least one target nucleic acid or target polypeptide in a sample of nucleic acids or polypeptides comprising the steps of: a) providing a sample comprising nucleic acids or polypeptides; b) labeling the nucleic acids or polypeptides with a ligand conjugate, the ligand conjugate comprising a first element binding to the nucleic acids or polypeptides and a second element which is a capture ligand; c) contacting the nucleic acid-ligand conjugates or polypeptide-ligand conjugates with at least one capture probe, the capture probe hybridizing with or binding to at least one target nucleic acid or target polypeptide; d) adding i) an oxidoreductase enzyme, wherein the oxidoreductase enzyme is recognized by the capture ligand, or ii) a complex comprising an oxidoreductase enzyme bound to a capture receptor, the capture receptor capable of binding to the capture ligand; e) adding a redox polymer, the redox polymer binding to the oxidoreductase enzyme, thereby resulting in the transfer of electrons from the enzyme via the redox polymer to an electrode surface; and f) detecting and/or quantifying the presence of the target nucleic acid(s) or target polypeptide(s).
REFERENCES:
patent: 2003/0082601 (2003-05-01), Dill
patent: 2004/0048241 (2004-03-01), Freeman et al.
patent: 0165246 (2001-09-01), None
patent: 03042396 (2003-05-01), None
patent: WO 03/042396 (2003-05-01), None
Xie et al (Nucleic Acids Research, 2004, 32(2):1-7).
Dequaire et al (Anal. Chem, 2002, 74:4370-4377).
Kainu et al (Cancer Research, Jul. 1996, 56:2912-2915).
Bardea et al., “Amplified microgravimetric quartz-crystal-microbalance analyses of oligonucleotide complexes: a route to a Tay-Sachs biosensor device”, Chemical Commununications, 1998, pp. 839-840.
Boon et al., “Mutation detection by electrocatalysis at DNA-modified electrodes”, Nature Biotechnology, 2000, vol. 18: 1096-1100.
Caruana et al., “Enzyme-Amplified Amperometric Detection of Hybridization and of a Single Base Pair Mutation in an 18-Base Oligonucleotide on a 7-um-Diameter Microelectrode”, Journal of the American Chemical Society, 1999, vol. 121 (4): 769-774.
Chan et al., “Nonisotopic Quantitation of mRNA Using a Novel RNase Protection Assay: Measurement of erbB-2 mRNA in Tumor Cell Lines”, Analytical Biochemistry, 1996, vol. 242 (2): 214-220.
Fotin et al., “Parallel thermodynamic analysis of duplexes on oligodeoxyribonucleotide microchips”, Nucleic Acids Research, 1998, vol. 26 (6): 1515-1521.
Jordan et al., “Surface Plasmon Resonance Imaging Measurements of DNA Hybridization Adsorption and Streptavidin/DNA Multilayer Formation at Chemically Modified Gold Surfaces”, Analytical Chemistry, 1997, vol. 69 (24): 4939-4947.
Kelley et al., “Electrochemistry of Methylene Blue Bound to a DNA-Modified Electrode”, Bioconjugate Chemical, 1997, vol. 8 (1): 31-37.
Kelley et al., “Single-base mismatch detection based on charge transduction through DNA”, Nucleic Acids Research, 1999, vol. 27 (24): 4830-4837.
Lay et al., “cis-BIS(2,2′-Bipyridine-N,N′) Complexes of Ruthenium(III)/(II) and Osmium(III)/(II)”, Inorganic Syntheses, 1986, vol. 24: 291-299.
Martin et al., “Linking Gene Expression Patterns to Therapeutic Groups in Breast Cancer”, Cancer Research, 2000, 60: 2232-2238.
Patolsky et al., “Amplified Microgravimetric Quartz-Crystal-Microbalance Assay of DNA Using Oligonucleotide-Functionalized Liposomes or Biotinylated Liposomes”, Journal of the American Chemical Society, 2000, vol. 122 (2): 418-419.
Rodriguez et al., “Electrochemical Studies of the Interaction of Metal Chelates with DNA. 4. Voltammetric and Electrogenerated Chemiluminescent Studies of the Interaction of Tris(2,2′-bipyridine)osmium(II) with DNA”, Analytical Chemistry, 1990, vol. 62(24): 2658-2662.
Sullivan et al., “Mixed Phosphine 2,2′-Bipyridine Complexes of Ruthenium”, Inorganic Chemistry, 1978, vol. 17 (12): 3334-3341.
Totzke et al., “Competitive reverse transcription/polymerase chain reaction for the quantification of p53 and mdm2 mRNA expression”, Molecular and Cellular Probes, 1996, vol. 10 (6): 427-433.
Trudeau et al., “Reagentless Mediated Laccase Electrode for the Detection of Enzyme Modulators”, Analytical Chemistry, 1997, vol. 69 (5): 882-886.
Xie et al., “Amperometric Detection of Nucleic Acid at Femtomolar Levels with a Nucleic Acid/Electrochemical Activator Bilayer on Gold Electrode”, Anal. Chem., vol. 76 (2004), pp. 1611-1617.
Xie et al., “Highly sensitive amperometric detection of genomic DNA in animal tissues”, Nucleic Acids Research, vol. 32, No. 2 (2004), pp. 1-7.
Wang, “Survey and Summary From DNA biosensors to gene chips”, Nucleic Acids Research, 2000, vol. 28 (16): 3011-3016.
Zhang et al., “Enzyme-Amplified Amperometric Detection of 3000 Copies of DNA in a 10-uL Droplet at 0.5fM Concentration”, Analytical Chemistry, 2003, vol. 75(13): 3267-3269.
Search Report for Taiwanese Patent Application 094113677.
Cottrez et al., “Quantitive PCR: validation of the use of a multispecific internal control”, Nucleic Acids Research, vol. 22, No. 13 (1994), pp. 2712-2713.
Decher, “Fuzzy Nanoassemblies: Toward Layered Polymeric Multicomposites”, Science, vol. 277 (1997), pp. 1232-1237.
International Search Report relating to PCT/SG 2004/000113.
Gao et al., “Electrodeposition of Redox Polymers and Co-Electrodeposition of Enzymes by Coordinative Crosslinking”, Angew. Chem. Int. Ed., vol. 41, No. 5 (2002).
Gao Zhiqiang
Xie Hong
Yu Yuan Hong
Aeder Sean E
Agency for Science Technology and Research
Caesar Rivise Bernstein Cohen & Pokotilow Ltd.
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