Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving urea or urease
Patent
1995-11-28
1998-07-28
Redding, David A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving urea or urease
4352841, 435818, A01N 102
Patent
active
057861366
DESCRIPTION:
BRIEF SUMMARY
The present invention relates to a method of and a device for conserving organs, body extremities resp lobes of body tissue and can specifically be applied for keeping organs, body extremities and lobes of body tissue vital at room resp body temperature. The invention can principally be applied in the fields of surgical transplantation, organ resp body tissue bank operation and research.
In clinical practice, it is known to "cold"-store free lobes of body tissue and isolated organs, which were extracted for transplantation into human beings, at 1.degree. to 6.degree. C. in cardioplegic solutions in order to reduce oxygen consumption and, thus, metabolism to a minimum. When doing so, free lobes of muscles, skin and/or bones shall be stored for a period not exceeding 24 hours before they are joined to a blood circulation again. In conjunction with animal experiments, maximum conservation periods of 45 hours have hitherto been attained.
These known solutions have the drawback of requisite cooling being laborious as well as the maximum conservation period being relatively short.
With organ conservation by means of hypothermal perfusion, it, furthermore, is known to make use of specific conservation solutions, such as are described in W. Konertz, Konservierung des Herzens zu Transplantationszwecken mit Euro-Collins-Lo sungen, Habilitation Thesis, Kiel, 1987. Dialysis procedures, such as have become known in connection with application of the man-made kidney, are likewise notorious.
With the known solutions, long-term conservation of organs, body extremities resp lobes of body tissue is not possible at room temperature.
It, therefore, is the object of the invention to provide a method and a device which enable effective and economic long-term conservation of organs, body extremities and lobes of body tissue at room temperature resp body temperature.
It, furthermore, is the object of the invention to improve the preconditions of efficient research and to minimise the number of requisite animal experiments.
In accordance with the invention, this object is solved by the features in the characterising part of claims 1 and 7 in conjunction with the features of the respective preambles. Practical developments of the invention can be found in the subclaims.
The special advantage of the invention resides in that long-term conservation of organs, body extremities or lobes of body tissue in the order of 8 days and more is made possible at room resp body temperature. At these temperatures, metabolism continues and preserves activity of the fibroplastics for wound healing and accretion. For instance, free lobes of body tissue can be caused to accrete through an extracorporal circulation system, and that without any microsurgical joining of vessels.
Oxygen supply and detoxication, therewith, are brought about through three discrete circulation systems, the first circulation system supplying the organs, body extremities resp lobes of body tissue with an oxygen-rich arterial perfusate and oxygenising as well as detoxicating the venous perfusate and exchanging metabolites; the physiological aqueous solution of the second circulation system being oxygenised in a dilution pot and delivering the oxygen on the dialysis membrane or before the latter to the first circulation system in the liquid phase and the third circulation system realising discontinuous cleaning-by-flushing of the organs resp lobes of body tissue with a physiological aqueous solution and the three circulation systems being controlled such that either only circulation systems 1 and 2 will jointly be in operation or circulation system 3 will be in operation.
As perfusate, aqueous nutritive solutions or blood or blood products may be used.
Another advantage of the invention resides in that animal experiments can be reduced because examinations can be carried out on isolated organs and blocks of body tissue, which, otherwise, would result in an animal experiment. It is also possible that several teams work with the same organs of an animal.
A standardised model for e
REFERENCES:
patent: 3738914 (1973-06-01), Thorne et al.
patent: 3772153 (1973-11-01), De Roissart
patent: 5472876 (1995-12-01), Fahy
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