Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-08-07
1998-06-30
Spiegel, Carol A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 72, 435 732, 435 792, 435 794, 435961, 435975, 436533, 436536, 436538, 436544, 436518, 436808, 436176, 436177, G01N 3353
Patent
active
057732345
ABSTRACT:
A lateral flow assay device for detecting the presence of Chlamydia antigen in patient's samples comprises a flow matrix including a labelling zone and a capture zone. Labelling complex comprising antibodies specific for an epitope on the lipopolysaccharide antigen of Chlamydia is present within the labelling zone. Immobilized antibody specific for the same or another epitope of the lipopolysaccharide antigen of Chlamydia is located in the capture zone. The sample containing the Chlamydia antigen will flow first through the labelling zone, where it complexes with the labelling complex, and then to the capture zone, where it is captured by the immobilized antibody. Chlamydia antigen may be extracted from a patient sample, such as a endocervical swab, by first extracting the antigen in a strong base followed by neutralization with a zwitterionic detergent and a blocking protein present in a zwitterionic buffer.
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Brade et al., "Chemical, Biological, and Immunochemical Properties of the Chlamydia psittaci Lipopolysaccharide," Infect. Immun. 54(2)568-74, Nov. 1986.
Klepper Robert E.
Pawlak Catherine
Pronovost Allan D.
Quidel Corporation
Spiegel Carol A.
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