Method and device for characterizing a culture liquid

Details Method and device for characterizing a culture liquid Method and device for characterizing a culture liquid

2005-06-14

2005-06-14

Witz, Jean C. (Department: 1651)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

C435S173100, C435S243000

Reexamination Certificate

active

06905838

ABSTRACT:
In a method for characterizing a culture liquid, in particular in a bioreactor, by illuminating the cells (22) contained in the culture liquid the culture liquid is analyzed in situ, microscopic imaging of the cells (22) and by evaluating the image. The illumination is carried out as dark-field illumination, and the image evaluation comprises a comparison of the intensities of the light output by the interior of the cell and by the edge of the cell, by means of which comparison living and dead cells (22) are distinguished in order to determine the vitality of the culture liquid.

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Korber et al. “Bacterial plamolysis as a physical indicator of viability” Appl. Environ. Microbiol. (Nov. 1996) 62(11): 3939-3847.
Copy of Search results from German priority patent application (in German).

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