Method and device for cell cultivation on electrodes

Details Method and device for cell cultivation on electrodes Method and device for cell cultivation on electrodes

1990-10-30

1992-07-28

Lacey, David L.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435284, 435287, 435297, 435289, 435310, 435817, 2041801, 204406, 204299R, 935 85, 935 52, 935 93, C12N 1300, B01D 5702, B01D 6142

Patent

active

051340704

ABSTRACT:
A method and device are disclosed for culturing of adherent cell monolayer cultures on an electrode surface and subjecting the cells to an electrical field. The device may generally comprises cell culture dish, for example a petri-type dish, having a bottom, with an electrically conductive, optically transparent coating amenable to cell adhesion on the upper surface of the bottom. A metal electrode may contact the underside of the coating and is connected to a source of electrical power. The method involves use of the device for culturing the cells under the intermittent or continuous influence of an electric field, or during the establishment of an electrical field or potential. Further, a method and device are disclosed for the sensing of cell poration, and the use of this information to automate the process of electroporation. Further yet, a device and method are disclosed for use in assisting in the time-correlated mapping of the sequence of serial and parallel gene replication.

REFERENCES:
patent: 4699881 (1987-10-01), Matschke
patent: 4804450 (1989-02-01), Mochizuki et al.
patent: 4818697 (1989-04-01), Liboff et al.
patent: 4923814 (1990-05-01), Marshall
Yaoita, et al., Exp. Cell. Biol., 57(1), pp. 43-51 (1989).

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