Method and compound for detecting low levels of microorganisms

Details Method and compound for detecting low levels of microorganisms Method and compound for detecting low levels of microorganisms

1998-12-04

2000-04-18

Gitomer, Ralph

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 63, 536 168, C12Q 104, G01N 3348, C07G 1100

Patent

active

060513950

ABSTRACT:
A method and compound for detecting low levels of microorganisms in biological samples is disclosed. In the method, an antibiotic is conjugated to a detectable label. This antibiotic/label conjugate is then introduced into a sample containing biological material. The antibiotic binds to target microorganism where the label allows for detection of localized concentrations of the antibiotic. A compound to accomplish this method is also described. This compound is an antibiotic conjugated to a fluorescent dye. This dye has an excitation and emission wavelength that are not interfered by substances typically found in biological samples.

REFERENCES:
patent: 4385126 (1983-05-01), Chen et al.
patent: 4661913 (1987-04-01), Wu et al.
patent: 5135851 (1992-08-01), Kajander
patent: 5137810 (1992-08-01), Sizemore et al.
patent: 5453505 (1995-09-01), Lee et al.
patent: 5545535 (1996-08-01), Roth et al.
patent: 5545721 (1996-08-01), Carroll et al.
patent: 5741657 (1998-04-01), Tsien et al.
patent: 5843699 (1998-12-01), Strenkoski et al.
Merriam Webster's Collegiate Dictionary, 10th edition, p. 50, Merriam-Webster, Inc. Springfield, MA. (1996). No Month Given.
Deng et al. Fluorescent conjugates of Brefeldin A selectively stain the endoplasmic reticulum and Golgi complex of living cells. J. Histochem. And Cytochem. 43(9), pp. 907-915. (Sep. 1995).
Drug Facts and Comparisons.RTM., 1995 Edition, pp. 2059, 2062, 2464. Facts and Comparisons, St. Louis, MO. (1995). No Month Given.
Read et al. Enumeration of CD34+ hematopoietic progenitor cells in peripheral blood and leukapheresis products by microvolume fluorimetry: a comparison with flow cytometry. J. Hematotherapy, 6(4), 291-301. (Aug. 1997). Abstract Only.
R. Bernander et al., "Flow Cytometry of Bacterial Cells: Comparison Between Different Flow Cytometers and Different DNA Stains", Cytometry 31:29-36 (1998).
M. J. Rybak et al., "Combination Antimicrobial Therapy for Bacterial Infections", Drugs Sep. 1996 52(3), 390-405.
J. R. Johnson et al., "Direct Antimicrobial Susceptibility Testing for Acute Urinary Tract Infections in Women", Journal of Clinical Microbiology, vol. 33, No. 9, Sep. 1995, pp. 2316-2323.
R. S. Pore, "Antibiotic susceptibility testing by flow cytometry", Journal of Antimicrobial Chemotherapy (1994) 34, 613-627.
J. L. Kadurugamuwa et al., "Surface Action of Gentamicin on Pseudomonas aeruginosa", Journal of Bacteriology, vol. 175, No. 18, Sep. 1993, pp. 5798-5805.
P. Schindler et al., "Action of Polymyxin B on Bacterial Membranes: Morphological Changes in the Cytoplasm and in the Outer Membrane of Salmonella typhimurium and Escherichia coli B", Antimicrobial Agents and Chemotherapy, Jul. 1975, pp. 95-104.
F. A. Chen et al., "Feasibility Studies for Simultaneous Immuno-chemical Multianalyte Drug Assay by Capillary Electrophoresis with Laser-Induced Fluorescence", Clinical Chemistry, vol. 40, No. 9 (1994), pp. 1819-1822.
B. A. Newton, "A Fluorescent Derivative of Polymyxin: its Preparation and Use in Studying the Site of Action of the Antibiotic", Journal of General Microbiology, vol. 12, No. 2 (1955), pp. 226-236.

Affiliated with

Also associated with

Rating

Method and compound for detecting low levels of microorganisms does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method and compound for detecting low levels of microorganisms, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method and compound for detecting low levels of microorganisms will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2335374