Method and compositions for identification of species origin of

Details Method and compositions for identification of species origin of Method and compositions for identification of species origin of

1996-05-13

1998-07-28

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 231, 536 243, 536 2433, C12Q 168, C12P 1934, C07H 2102, C07H 2104

Patent

active

057861447

ABSTRACT:
The present invention provides a method and compositions for species identification from small samples of fish tissue. The method includes the use of the polymerase chain reaction (PCR) to amplify regions of the mitochondrial genome from total cellular DNA with species-specific primers and subsequent analysis of the PCR products. The method provides an accurate and rapid determination of the species of origin for a single egg of processed caviar. Compositions for PCR primers specific for 27 species of sturgeon are provided.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
Birstein, et al. Tetraploid-Octoploid relationships and karyological evolution in the order Acipenseriformes (Pisces) Karyotypes, nucleoli, and nucleolus-organizer regions in four aceipenserid species, Genetica,vol. 73, pp. 3-12 (1987).
Walsh, et al., "Chelex 100 as a Medium for Simple Extraction of DNA for PCR-Based Typing from Forensic Material", Biotechniques, vol. 10, No. 4, pp. 506-513 (1991).
Keyvanfan, A.C, et al C.R. Acad Sc. Paris Ser III No. 9, vol. 304, pp. 191-193, 1987.
Baker et al., "Which whales are hunted? A molecular genetic approach to monitoring whaling", American Assoc. for the Advancement of Science, Science, vol. 265, pp. 1538-1539.
Birstein, V.J., "Sturgeons and Paddlefishes: Threatened Fishes in Need of Conversation", Conserv. Biol., vol. 7, pp. 773-787 (1993).
Brown, J.R., et al., "Nucleotide Sequence of the apocytochrome B Gene in White Surgeon Mitochondrial DNA", Nucleic Acids Research, vol. 17 (11), p. 4389 (1989).
Brown, J.R., et al., "Length Variation, Heteroplasmy and Sequence Divergence in the Mitocondrial DNA of Four Species of Sturgeon (Acipenser)", Genetics, vol. 142, pp. 525-535 (1996).
Cronin, et al., Wildlife Society Bulletin, vol. 19, pp. 94-105 (1991).
DeSalle, R. et al., "Molecular Evolution: Producing the Biochemical Data", Methods in Enzymology, vol. 224, pp. 176-204 (1993).
Gardiner, B.G., "Sturgeons as Living Fossils", Living Fossils, eds. Eldredge, N. & Stanley, S.M., Living Fossils, pp. 148-152 (1984).
Graves, et al., "Biochemical Genetics of Southern California Basses of the Genus Paralabrax: Specific Identification of Fresh and Ethanol-preserved individual Eggs and Early Larvae", Fishery Bulletin, vol. 88, pp. 59-66 (1989).
Hedges, et al., "Caecilian Phylogeny and Biogeography Inferred from Mitochondrial DNA Sequences of the 12S rRNA and 16S rRNa Genes (Amphibia: Gymnophion A)", Herpetological.
Kocher, et al., "Dynamics of mitochondrial DNA evolution in animals: Amplificatin and sequencing with conserved primers", Proceedings of the Natl. Academy of Science of the USA, vol. 86, pp. 6169-6200 (1989).
Meyer, et al., "Origin of Tetrapods Inferred from Their Mitochondrial DNA Affiliation to Lungfish", J. Mol. Evol., vol. 31, pp. 359-364 (1990).
Milner-Gulland and Mace, "The Implact of the Ivory Trade on the African Elephant Loxodonta africana Population as Assessed by Data from the Trade", Biological Conservation, vol. 55.
Miracle, A.L. & Campton, D.E., "Tandem Repeat Sequence Variation and Length Heteroplasmy in the Mitochondrial DNA D-Loop of the Threatened Gulf of Mexico Sturgeon, Acipenser oxyrhynchus desotoi", Journal of Heredity, vol. 86(1), pp. 22-27 (1995).
Ong, T.L., et al., "Genetic Divergence between Acipenser oxyrinchus oxyrichus and A. o. desotoi as Assessed by Mitochondrial DNA Sequencing Analysis", COPEIA 2, (1996).
Rehbein, H.Z., "Caviare: Proximate Composition, Amino Acid Content and Identification of Fish Species", LEBENSM, Unters, Forsch, vol. 180, pp. 457-462 (1985).
Amato et al., "PCR assays of variable nucleotide sites for identification of conservation units," in Molecular Approaches to Ecology and Evolution, Schierwater, B, et al., eds.
Birstein, et al., Genetica, vol. 73, pp. 3-12 (1993).
Palumbi, et al., The Simple Fool's Guide to PCR, Biol. 101, Version 2 (1991).
Walsh, et al., Bio. Techniques, vol. 10, pp. 506-513 (1991).
Keyvanfar A. Am Inst. Oceanog? (Paris) vol. 64 pp. 25-64, 1988 for considered Feb. 16, 1998.
Brown et al. Genetics 142: 525-535, 1996.
DeSalle et al. Methods in Enzymology 224: 176-204, 1993.
Hara et al. Bulletin of the Japan Sea NAtional Fisheries Research Institute (Abstract provided) vol. 44: 131-138, 1994.

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