Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1995-05-11
1998-08-04
Low, Christopher S. F.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4351722, 4351736, 435173, 435320, 435325, 514 44, C12N 1300
Patent
active
057892130
ABSTRACT:
Methods and compositions are provided for use in electroloading procedures to increase the transfection and fusion efficiency compared to the methods now used in the art. The compositions comprise a two-phase polymer system containing two water soluble polymers which, when mixed, result in target cells and biological material being encapsulated into one of the polymer phases in a concentrated form. The methods of the present invention for electroloading biological material into target cells comprises mixing the biological material into one of the phases of the two-phase polymer system; mixing the target cells into either of the phases of the two-phase polymer system; mixing the phases together to form an emulsion; and exposing the emulsion to a pulsing electric field in an electroloading process.
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Hui Sek Wen
Stoicheva Natailia
Zhao Ya-Li
Health Research , Inc.
Low Christopher S. F.
Nguyen Dave
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