Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-10-11
1998-04-07
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 5, 435 912, 435 71, 435 72, 435 79, 435210, 536 243, C12P 168, C12P 1934, C12Q 170, C07H 2104
Patent
active
057363307
ABSTRACT:
A method for the analysis of DNA sequences and PCR products comprises the steps of constructing an oligonucleotide-labeled bead set, and labeled complementary probe, and exposing the bead set and probe to a DNA fragment or PCR product under hybridizing conditions and analyzing the combined sample/bead set by flow cytometry. Flow cytometric measurements are used to classify beads within an exposed bead set to determine the presence of identical or nonidentical sequences within the test sample. The inventive technology enables the rapid analysis of DNA sequences and detection of point mutations, deletions and/or inversions while also reducing the cost and time for performing genetic assays.
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Luminex Corporation
Rees Dianne
Zitomer Stephanie W.
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