Method and compositions for determination of gamma glutamyl tran

Details Method and compositions for determination of gamma glutamyl tran Method and compositions for determination of gamma glutamyl tran

1983-06-08

1985-12-24

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 24, 435188, 435810, C12Q 148, C12Q 136, C12N 996

Patent

active

045606501

ABSTRACT:
A two-reagent assay system for determination of gamma glutamyl transpeptidase enzyme activity in a biological fluid. Gamma glutamyl-p-nitroanalide substrate for the enzymatic reaction is provided in a first reagent which is a dry mixture of the substrate with a normally solid acid which is water soluble and a second reagent includes a buffer and a component selected for reception of the glutamyl product of the enzymatic reaction.

REFERENCES:
patent: 3703441 (1972-11-01), Nakanishi et al.
patent: 3892631 (1975-07-01), Carroll
patent: 3986931 (1976-10-01), Bernt et al.
patent: 4087331 (1978-05-01), Bucolo et al.
patent: 4372874 (1983-02-01), Modrovich
Rosalki et al., Clin. Chem., 20(9): 1121-1124 (1974).

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