Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1997-08-27
1998-07-21
Ketter, James
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4353201, 536 234, 536 2353, 536 241, C12P 2102, C12N 1563, C12N 1513
Patent
active
057834202
ABSTRACT:
The present invention is directed to methods and compositions useful for altering the transcriptional expression of genes in eukaryotic cells. The invention employs novel antibody derivative molecules which function to recognize and bind to specific cis-regulatory DNA sequence elements of a eukaryotic gene. When two antibody derivative molecules are bound to adjacent cis-regulatory DNA sequence elements of a gene, those molecules may interact to form an antibody binding site which is capable of recognizing and binding to a transcription factor protein for the target gene, thereby affecting the functionality of that transcription factor protein and, in turn, the transcriptional activity of the gene. Also provided herein are isolated nucleic acids encoding the novel antibody derivative molecules of the present invention and expression vectors comprising those nucleic acids.
REFERENCES:
Beerli, R.R., et al., "Autocrine Inhibition of the Epidermal Growth Factor Receptor by Intracellular Expression of a Single-Chain Antibody," Biochemical and Biophysical Research Communications 204(2):666-672 (1994).
Beerli, R.R., et al., "Intracellular Expression of a Single Chain Antibodies Reverst ErbB-2 Transformation," The Journal of Biological Chemistry, 269(39):23931-23936 (1994).
Biocca, S., et al., "Expression and Targeting of Intracellular Antibodies in Mammalian Cells," The EMBO Journal, 9(1):101-108 (1990).
Kabat, E.A., Recombination of Separated Chains to Form IgG, Structural Concepts in Immunology and Immunochemistry (2nd Ed.), Holt, Rinehart ad Winston: New York (1976) pp. 248-252.
Marasco, W.A., et al., "Design, Intracellular Expression, and Activity of a Human Anti-Human Immunodeficiency Virus Type 1 gp 120 Single-Chain Antibody," Proc. Natl. Acad. Sci. USA, 90:7889-7893 (1993).
Brusca John S.
California Institute of Technology
Ketter James
LandOfFree
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