Method and composition for vitamin B-12 Assay

Details Method and composition for vitamin B-12 Assay Method and composition for vitamin B-12 Assay

1980-05-15

1982-06-01

Nucker, Christine M.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

23230B, 424 12, G01N 3354, G01N 3358, G01N 3360

Patent

active

043327869

ABSTRACT:
The binding of vitamin B-12 by natural substances in samples assayed for vitamin B-12 is reduced by heating the test sample in an alkaline buffer comprising thioglycerol and denaturing agent having the formula (R).sub.2 NC(A)N(R).sub.2, wherein R is hydrogen or lower alkyl and A is oxygen or hydroxyl.

REFERENCES:
patent: 3937799 (1976-02-01), Lewin et al.
patent: 4028465 (1977-06-01), Lewin et al.
patent: 4146602 (1979-03-01), Gutcho et al.
patent: 4188189 (1980-02-01), Allen
Ithakissios, et al. "Clin. Chem." 26(2): 323-326, Feb. 1980.
"Chemical Abstracts" 87:806185 (1977).
Fox, et al., Introduction to Protein Chemistry, pp. 304-326 (1957).

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