Method and composition for the enzymic determination of aspartam

Details Method and composition for the enzymic determination of aspartam Method and composition for the enzymic determination of aspartam

1995-11-07

1997-11-11

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 4, 435 16, 435 24, 435 25, 435 26, 435288, 435808, 435810, C12Q 137, C12Q 100

Patent

active

056862601

ABSTRACT:
The invention relates to a method and a composition for the enzymic determination of aspartame in aqueous solutions. The method is carried out such that the sample solution is incubated essentially in the presence of oxo-glutarate, glutamate-oxaloacetate transaminase (GOT), malate dehydrogenase (MDH), NADH and a protease, and the decrease in the NADH extinction is measured photometrically.

REFERENCES:
patent: 5279945 (1994-01-01), Hummel
patent: 5429726 (1995-07-01), Johnson et al.
Boki et al, Jpn. J. Toxicol. Environm. Health, vol. 39, No. 1, pp. 72-75, 1993.
Mulchandani et al, Analytica Chimica Acta, vol. 234, pp. 465-469, 1990.
Boki et al, Chemical Abstracts, vol. 118, p. 755, Ref. #167757u, 1993 (Jpn. J. Toxicol. Environ. Health 1993 39(1) 72-75).

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