Method and composition for the determination of glycerol

Details Method and composition for the determination of glycerol Method and composition for the determination of glycerol

1979-07-05

1981-06-16

Hruskoci, Peter A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435190, C12Q 132

Patent

active

042738709

ABSTRACT:
Method and composition for the determination of glycerol using glycerin dehydrogenase, an electron transfer agent, NAD and a tetrazolium salt and, in addition, at least one microgram of zinc, in the form of a zinc salt, per 50 U of glycerol dehydrogenase.

REFERENCES:
patent: 3703591 (1972-11-01), Bucolo et al.
patent: 3862009 (1975-01-01), Wahlefeld et al.
patent: 4001089 (1977-01-01), Stavropoulos et al.
patent: 4045297 (1977-08-01), Weeks et al.
patent: 4142938 (1979-03-01), Stavropoulos et al.
Lin et al., "The Activation of Glycerol Dehydrogenase from Aerobacter aerogenes by Monovalent Cations, " Journal of Biol. Chem., Jun. 1960, pp. 1820-1828.

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