Method and composition for the determination of cholesterol

Details Method and composition for the determination of cholesterol Method and composition for the determination of cholesterol

1981-12-07

1985-03-05

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving cholesterol

435 19, 435 25, 435 28, 435805, 435810, 435886, C12Q 160, C12Q 144, C12Q 126, C12Q 128, C12R 1465

Patent

active

045031444

ABSTRACT:
The present invention provides a process for the determination of cholesterol in free or bound form by means of cholesterol oxidase and optionally of cholesterol esterase by measurement of the oxygen consumption, of the hydrogen peroxide formed or of the cholestenone formed, wherein the determination is carried out kinetically with the use of a cholesterol oxidase which has been obtained from a micro-organism of the genus Streptomyces and with the addition of 3,4-dichlorophenol.
The present invention also provides a reagent for the kinetic determination of cholesterol, comprising cholesterol oxidase and a system for the determination of hydrogen peroxide or of cholestenone and optionally cholesterol esterase, wherein it additionally contains 3,4-dichlorophenol and the cholesterol oxidase used originates from a micro-organism of the genus Streptomyces.

REFERENCES:
patent: 3983005 (1976-09-01), Goodhue et al.
patent: 4212938 (1980-07-01), Gruber et al.
patent: 4226713 (1980-10-01), Goldberg
patent: 4229527 (1980-10-01), Ziegenhorn et al.
patent: 4350762 (1982-09-01), DeLuca
Otani et al., Chemical Abstracts, 87: 80812a, 257, (1977).
Otani et al., Chem. Pharm. Bull., 25(6): 1452-1455, (1977).
Tomioka et al., J. Biochem., 79: 903-915, (1976).

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