Method and composition for lyophilizing red blood cells

Details Method and composition for lyophilizing red blood cells Method and composition for lyophilizing red blood cells

1996-06-19

1998-05-12

Saucier, Sandy

Chemistry: molecular biology and microbiology

Maintaining blood or sperm in a physiologically active state...

435 13, A01N 102

Patent

active

057503303

ABSTRACT:
Disclosed are a composition for the lyophilization of mammalian red blood cells comprising a hydrophilic polymer, a carbohydrate, and an organic solvent; and a method of using the composition to lyophilize red blood cells comprising mixing red blood cells with the composition, freezing the mixture, and drying the mixture by removing water by sublimation. Also disclosed are red blood cells lyophilized according to this method for lyophilization, and a method for reconstituting the lyophilized red blood cells. In particular, the composition used to lyophilize the red blood cells comprises a mixture of a hydrophilic polymer ranging from 1,450-20,000 Daltons at 5-50% w/v, a mono- or disaccharide or a mixture thereof from 0.01-0.2M and an organic solvent such as a primary alcohol, a secondary alcohol, dimethyl sulfoxide or combinations thereof at 0.5-20% v/v.

REFERENCES:
patent: 4874690 (1989-10-01), Goodrich, Jr. et al.
patent: 5043261 (1991-08-01), Goodrich et al.
patent: 5045446 (1991-09-01), Goodrich, Jr. et al.
patent: 5171661 (1992-12-01), Goodrich, Jr. et al.
patent: 5242792 (1993-09-01), Rudolph et al.
patent: 5340592 (1994-08-01), Goodrich, Jr. et al.
patent: 5364756 (1994-11-01), Livesey et al.
patent: 5425951 (1995-06-01), Goodrich, Jr. et al.
Jones RC, Aust. J. Biol. Sci 22:983-94(1969).
Riazantsev V et al., Ukrainskii Biokhimichskii Zhurnal 59(5):97-9 (1987).

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