Method and composition for internal identification of samples

Details Method and composition for internal identification of samples Method and composition for internal identification of samples

1994-12-22

1998-07-07

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 912, 435 6, 435 5, 435174, 4351731, 4351723, 4353201, 4352523, 536 243, 536 2433, 536 2432, 2504581, C12P 1934, C12Q 168, C12Q 170, C07M 2104

Patent

active

057767378

ABSTRACT:
Patient samples are identified by adding to the sample, preferably at the time it is taken, a plurality of identification oligonucleotides. The identification oligonucleotides are co-processed and sequenced at the same time as the sample. The resulting sequence analysis thus provides both the sequence of the region of interest in the sample DNA, and the sequence of the identification oligonucleotides which are used to confirm the identity of the patient. In an embodiment of the invention, a plurality of specially constructed identification oligonucleotides is used. Each identification oligonucleotide is constructed based upon a starting oligonucleotide and comprises
(a) a primer site which is not homologous with DNA from the organism from which the sample is taken and which may be the same or different from the primer site of the other identification oligonucleotides; and
(b) an identification region having the general formula

REFERENCES:
patent: 5139812 (1992-08-01), Lebacq
patent: 5451505 (1995-09-01), Dollinger
patent: 5491225 (1996-02-01), Picone et al.
patent: 5650274 (1997-07-01), Kambara et al.
Bhat, Gene 134:83-87, 1993.

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