Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Patent
1996-10-17
1998-06-23
Leary, Louise N.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
435 18, 435 4, 435 17, 435 23, 435 24, 435886, 435897, 4352401, C12Q 148, C12Q 134, C12Q 137, C12N 100
Patent
active
057703922
ABSTRACT:
Materials can be assayed for activity as an inhibitor of post-translational protein phosphorylation by adding the material to a growing culture of a prokaryotic organism such as a streptomycete; allowing the culture to grow for a period of time in the presence of the material; and observing the culture for altered development relative to development of the prokaryotic organism grown in the absence of the material. Observation of altered development is indicative that the material has activity as an inhibitor of post-translational protein phosphorylation. In particular, the material to be tested can be added to a growing culture of the prokaryotic organism by placing a carrier disk bearing the material on a freshly seeded plate. Inhibition of the development of aerial mycelia and spore formation is an indicator that the material has activity as an inhibitor of post-translational protein phosphorylation.
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Davies Julian E.
Waters Barbara
Leary Louise N.
TerraGen Diversity Inc.
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