Method and composition for determining an oxidized pyridine co-e

Details Method and composition for determining an oxidized pyridine co-e Method and composition for determining an oxidized pyridine co-e

1979-07-19

1981-07-14

Wyse, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving luciferase

435 26, C12Q 166

Patent

active

042787602

ABSTRACT:
A method for the quantitative determination of an oxidized pyridine co-enzyme which comprises reacting a sample containing oxidized pyridine co-enzyme in the presence of its specific alcohol dehydrogenase with an aliphatic alcohol of 8 to 16 carbon atoms, oxidizing the aldehyde formed with reduced flavine mononucleotide and oxygen in the presence of luciferase and of the particular FMN-reductase which is independent of the co-enzyme to be determined and of the reduced substrate co-enzyme of the latter, and measuring the resulting light emission as a measure of the initial oxidized pyridine co-enzyme content.

REFERENCES:
patent: 3331752 (1967-07-01), Struck
patent: 3660240 (1972-05-01), Chappelle et al.
patent: 3941659 (1976-03-01), Koch et al.
patent: 3953295 (1976-04-01), Monte et al.
patent: 4097338 (1978-06-01), Konttinen et al.
patent: 4202938 (1980-05-01), Haeckel et al.
patent: 4218536 (1980-08-01), Maurukas
patent: 4229527 (1980-10-01), Ziegenhorn et al.
patent: 4229529 (1980-10-01), Michal et al.

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