Method and composition for determination of glycerol

Details Method and composition for determination of glycerol Method and composition for determination of glycerol

1984-04-17

1987-01-13

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 19, 435 26, 435810, C12Q 148, C12Q 144, C12Q 132

Patent

active

046364650

ABSTRACT:
A method for determination of glycerol by using a reagent system comprising glycerol dehydrogenase and a pyridine nucleotide coenzyme, characterized in that an enzyme selected from the group consisting of triokinase and dihydroxyacetone kinase is incorporated into the reagent system to eliminate the formed dihydroxyacetone or D-glyceraldehyde in the assay system.

REFERENCES:
patent: 3703591 (1972-11-01), Bucolo et al.
patent: 4273870 (1981-06-01), Mollering et al.
Lerner et al, Biochim. Biophys. Acta, 615: 1-9 (1980).
Boobis et al, Chemical Abstracts, 99:118623c, 322 (1983).
Hers, Methods in Enzymology, vol. V, Colowick et al (ed)., Academic Press, New York, 362-364 (1962).
Weinhouse et al, J. Bact., 127(2): 747-754 (1976).

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