Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1998-09-18
2000-02-08
Witz, Jean C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 30, 435219, 435184, C12Q 137, C12Q 124, C12N 999, C12N 950
Patent
active
060226986
ABSTRACT:
A composition for use in a medium for detecting yeasts and molds in a test sample, the composition comprises a substrate for an aminopeptidase; and a signal moiety linked to the substrate, the signal moiety capable of providing a detectable signal when cleaved by aminopeptidase in yeasts or molds. Optionally, an aminopeptidase inhibitor is added to the composition, to reduce endogenous enzymatic activity in samples. A method for use of the composition to detect the presence or amount of yeasts and/or molds in a test sample comprises inoculating a medium comprising the composition of claim 1 with said test sample; incubating the inoculated medium under conditions suitable for the growth of yeasts or molds; and, observing any detectable signal, whereby the presence of a detectable signal indicates the presence of yeasts or molds in the test sample.
REFERENCES:
patent: 4675289 (1987-06-01), Kanou et al.
patent: 5073488 (1991-12-01), Matner et al.
patent: 5429933 (1995-07-01), Edberg
Chen Chun-Ming
Gu Haoyi
Idexx Laboratories Incorporated
Witz Jean C.
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