Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1996-12-19
1998-12-29
Witz, Jean C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 30, 435219, 435184, C12Q 137, C12Q 124, C12N 999, C12N 950
Patent
active
058540113
ABSTRACT:
A composition and method for detecting the presence or amount of yeasts and molds in a test sample is presented. The composition contains a substrate and an inhibitor for an aminopeptidase. The substrate has a signal moiety capable of providing a detectable signal when cleaved by an aminopeptidase in yeasts or molds. The aminopeptidase inhibitor serves to reduce the endogenous aminopeptidase activity in the test sample. The method to detect yeasts or molds in a sample includes inoculating a test sample with the disclosed composition, incubating the sample and observing any detectable signal that indicates the presence of yeasts or molds.
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Chen Chun-Ming
Gu Haoyi
Hanley Susan
Idexx Laboratories Incorporated
Witz Jean C.
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