Method and cloning vectors utilizing intergeneric...

Chemistry: molecular biology and microbiology – Vector – per se

Reexamination Certificate

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C435S069100, C435S252300, C435S252330, C435S252400

Reexamination Certificate

active

07608448

ABSTRACT:
The present invention relates to a vector comprising sequences that permit direct transfer of the vector from one prokaryotic cell to another, such as by intergeneric conjugation. The invention also relates to methods of making and using the vector.

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patent: 2002/0015989 (2002-02-01), Hosted, Jr. et al.
patent: 0 620 280 (1994-10-01), None
patent: WO 99/00517 (1999-01-01), None
Bailey et al, Properties of in vitro recombinant derivatives of pJV1, a multicopy plasmid from Streptomyces phaeochromogenes, J Gen Microbiol, 1986, 132(8), pp. 2071-2078.
Baltz et al., Molecular genetic methods for improving secondary metabolite production in Actinomyctes. Trends Biotechnol. 1996; 14: 245-50.
Flett et al., High efficiency intergeneric conjugal transfer of plasmid DNA fromEscherchia colito methyl DNA-restricting streptomycetes. FEMS Micorbiol. Lett. 1997; 155: 223-9.
Mazodier et al., Intergeneric conjugation betweenEscherichia coliand Streptomyces species. J. Bacteriol. 1989; 171: 3583-85.

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