Method and chemical sensor for determining concentrations of hyd

Electrolysis: processes – compositions used therein – and methods – Electrolytic analysis or testing – For oxygen or oxygen containing compound

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2057775, 204403, 204416, 204419, G01N 2726

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active

060427140

ABSTRACT:
A new method which employs a mixed-valence cluster of M.sub.y.sup.z+ [Fe(II)(CN).sub.6 ] coated on an electrode surface to determine hydrogen peroxide concentration electrochemically is developed. M of the mixed-valence compound can be Co, Ni, Cr, Sc, V, Cu, Mn, Ag, Eu, Cd, Zn, Ru or Rh; z is the valence state of M; and y=4/z. In addition, this invention also reveals a new approach to determine a concentration of a hydrogen peroxide precursor, wherein a catalyst is immobilized in the matrix or on the surface of the mixed-valence compound on the electrode. In a typical biochemical system, the catalyst can be a glucose oxidase and blood sugar is catalyzed to form hydrogen peroxide.

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Chen-Xin et al. Catalytic oxidation of reduced nicotinamide dinucleotide at a microband gold electrode modified with nickel hexacyanoferrate. Analytica Chimica Acta, vol:310, Iss. 1, Jun. 1995.
Milardovic et al. Glucose determination in blood samples using flow injection analysis and an amperometric biosensor based on glucose oxidase immobilized on hexacyanoferrate modified nickel electrode. Analytica Chimica Acta, vol:350, Iss. 1-2, Sep. 1997.
Chen-Xin et al. Cobalt Hexacyanoferrate modified microband gold electrode and its electrocatalytic activity for oxidation of NADH. Journal of Electroanalytical Chemistry, vol:397, Iss. 1-2, Nov. 1995.

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