Method and cell line for testing mutagenicity of a chemical

Details Method and cell line for testing mutagenicity of a chemical Method and cell line for testing mutagenicity of a chemical

1991-06-27

1993-01-19

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

4351723, 4352402, C12Q 102, C12N 522, C12N 1512, C12N 1509

Patent

active

051806665

ABSTRACT:
A method of testing the mutagenicity of a chemical includes the steps of exposing test cells to the chemical in vitro, intracellularly metabolizing the chemical into a mutagenic or cytotoxic metabolite and then detecting gene/protein/cell damage in the test cells as an indication of the mutagenicity/cytotoxicity of the chemical.
A cell line is provided for testing mutagenicity of the chemicals, the cell line consisting essentially of fibroblasts normally having no detectable cytochrome P450 mixed function oxidase enzyme activity. The fibroblasts are transformed with chimeric gene constructs containing cytochrome P450 coding sequences and have intracellular cytochrome P450 oxidative metaboli zing activity.

REFERENCES:
patent: 4696898 (1987-09-01), Fitts et al.
Dogra et al., Mol. Pharmacol. vol. 37, 1990, pp. 608-613.
Crespi et al., Carcinogenesis vol. 10, 1989, pp. 295-301.
Kraemer et al., PNAS, vol. 72, 1975, pp. 59-63.
Govan et al., NAR, vol. 18, 1990, pp. 3823-3830.
Hoy et al., Chem. Abstr. vol. 102, 1985, abstract No. 19335.

Affiliated with

Also associated with

Rating

Method and cell line for testing mutagenicity of a chemical does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method and cell line for testing mutagenicity of a chemical, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method and cell line for testing mutagenicity of a chemical will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-101774