Method and cell line for testing cytotoxicity and mutagenicity o

Details Method and cell line for testing cytotoxicity and mutagenicity o Method and cell line for testing cytotoxicity and mutagenicity o

1994-11-15

1996-06-11

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 29, 4351723, C12Q 118, C12N 510, C12N 1509

Patent

active

055254829

ABSTRACT:
A method of testing the cytotoxicity and mutagenicity of a chemical includes the steps of exposing test cells to the chemical in vitro, intracellularly metabolizing the chemical into a mutagenic or cytotoxic metabolite and then detecting gene/protein/cell damage in the test cells as an indication of the mutagenicity/cytotoxicity of the chemical.
A cell line is provided for testing cytotoxicity and mutagenicity of the chemicals, the cell line consisting essentially of fibroblasts normally having no detectable cytochrome P450 mixed function oxidase enzyme activity. The fibroblasts are transformed with chimeric gene constructs containing cytochrome P450 coding sequences and have intracellular cytochrome P450 oxidative metabolizing activity.

REFERENCES:
patent: 4696898 (1987-09-01), Fitts et al.
patent: 5164313 (1992-11-01), Gelboin et al.
patent: 5180666 (1993-01-01), States et al.
McManus et al., Cancer Res., v. 50, Jun./1990, pp. 3367-3376.
Crespi et al., Toxicology, v. 82, 1993, pp. 89-104.
Perkus et al., J. Virol., vol. 63, 1989, pp. 3829-3836.
Hay et al., Mutat. Res., vol. 130, 1984, pp. 321-322.
Webster's Ninth New Collegiate Dictionary, 1990, Merriam-Webster Inc., Springfield, MA, p. 412.
B. Ames et al: Science 176:47-49 (1972).
B. Ames et al: Mutat. Res. 31:347-364 (1975).
A. Akeson et al., J. Biol. Chem. 263:16291-16296 (1988).
V. Bohr et al., Cell 40:359-369 (1985).
J. Cleaver et al., Ann Rev. Genet 9:19-38 (1975).
J. Cleaver et al., Cancer Res. 33:362-369 (1973).
C. Crespi et al., Carcinogenesis 10:295-301 (1989).
R. Davies et al., Carcinogenesis 10:885-891 (1989).
J. Doehmer et al., Proc. Natl. Acad. Sci (USA) 85:5769-5773 (1988).
S. Dogra et al., Molec. Pharmacol. 37:608-613 (1990).
F. Gonzalez et al., Mutation Res. 247:113-127 (1991).
F. Guengerich et al., Biochemistry 21:6019-6030 (1982).
H. Govan III et al., Nucleic Acids Res 18-3823-3830 (1990).
D. Hamer et al., J. Mol. & Appl. Gen. 1:273-288 (1982).
P. Iversen et al., Arch. Biochem. Biophy. 256:397-401 (1987).
K. Kraemer et al., Proc. Natl. Acad. Sci. (USA) 72:59-63 (1975).
W. Kleijer et al., Mutat. Res. 9:517-523 (1970).
A. Klotz et al., Anal. Biochem. 140:138-145 (1984).
R. Lubet et al., Mutat. Res. 142:127-131 (1985).
T. McLemore et al., J. Nat'l. Cancer Inst. 82:1333-1339 (1990).
A. Miller, Anal. Biochem. 133:46-57 (1983).
D. Nebert et al., DNA 6:1-11 (1987).
D. Nebert et al., DNA 8:1-13 (1989).
M. Pasanen et al., Pharmacol & Toxicol. 62:311-317 (1988).
A. Puga et al., DNA and Cell Biol. 9:425-436 (1990).
D. Thomas et al., Proc. Nat'l Acad. Sci. (USA) 85:3723-3727 (1988).
L. Thompson, Mutation Res. 247:213-219 (1991).
J. States, Mechanisms & Cons. of DNA Damage Proc., UCLA Symposia on Molecular & Cellular Biology, New Series, 83:307-311 (1988).
J. van Gurp et al., Mol. Cell Biol. 4:1597-1604 (1984).

Affiliated with

Also associated with

Rating

Method and cell line for testing cytotoxicity and mutagenicity o does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method and cell line for testing cytotoxicity and mutagenicity o, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method and cell line for testing cytotoxicity and mutagenicity o will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-351014