Method and cell line for obtaining plasminogen activators

Details Method and cell line for obtaining plasminogen activators Method and cell line for obtaining plasminogen activators

1985-02-22

1988-07-12

Moskowitz, Margaret

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 70, 4352402, 43524031, C12P 2100, C12N 500

Patent

active

047570059

ABSTRACT:
A method of increasing the yield of plasminogen activator from a plasminogen activator-producing cell line by adapting said cell line to grow in a suitable growth medium essentially free of fetal calf serum is disclosed. Also disclosed are cell lines capable of producing relatively large quantities of plasminogen activator, and a chemically defined growth medium suitable for adapting a plasminogen activator-secreting cell line to growth in the absence of fetal calf serum.

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