Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals
Patent
1992-10-30
1994-08-16
Scheiner, Toni R.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
422 58, 422 61, 422 681, 422102, 435 72, 435 725, 436165, 436514, 436519, 436520, 436524, 436527, 436528, 436529, 436534, 436536, 436538, 436541, 436805, 436808, 436809, 436824, G01N 33537, G01N 33538, G01N 33555, G01N 33558
Patent
active
053386892
ABSTRACT:
A method of detecting target antibodies or antigens by reaction with specific binding partners thereto is disclosed. One of the target or the binding partner is bound to a carrier, and the other is unbound. The complex between the target and the binding partner, with one being carrier-bound, forms an optically detectable binding complex. A microreaction vessel having an upper portion, a transition portion and a lower portion is utilized, wherein the upper portion has a greater diameter or width than the lower portion, and the transition portion is situated between the upper portion and the lower portion, and is funnel shaped. The microreaction vessel contains a slurry or suspension of inert particles, and unbound target or binding partner thereto. A solution of the carrier bound target or binding partner thereto is added to the vessel, which is then centrifuged to produce an optical determination of the target. A strongly positive reaction is indicated by a complex lying upon or in a top portion of a layer inert particles, a weakly positive reaction is indicated by a complex located within a lower portion of the layer of inert particles, and a negative reaction is indicated by the absence of a complex, with the carrier bound material lying beneath the inert particles.
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Adam Jean
Greber-Widmer Susanne
Josef Dieter
Yves Lapierre
Chin Christopher L.
Scheiner Toni R.
Stiftung Fur Diagnostische Forschung
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