Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1979-10-29
1982-10-05
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435241, 435284, 435285, 435286, C12M 300, C12N 500, C12N 502
Patent
active
043528872
ABSTRACT:
A method, solid-state culture support and culture solution are disclosed which enables successful in vitro culturing of differentiated cells, with significant retention of their differentiated character. Through the use of extra-cellular matrix fibers, specifically derived from connective tissue, as culture substrates, the method also discloses the isolation of the connective tissue fibers and their preparation as a culture substrate. This method provides significantly higher survival and attachment rates, and often significantly improved growth properties for in vitro cultures of differentiated cells, especially epithelial, over the current methods for culturing these cells.
This method also significantly enables certain differentiated cells to retain much of their normal enzymatic activities.
Furthermore, this method enables certain differentiated cells to retain to a high degree, their ability to secrete substances, such as hormones.
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Reid et al., Methods in Enzymology, Cell Culture, vol. LVIII, Academic Press, New York, 263-278, (1979).
Hauschka, Growth, Nutrition and Metabolism of Cells in Culture, Academic Press, New York, 90-104, (1972). vol. II.
Reid Lola C. M.
Rojkind Marcos
Albert Einstein College of Medicine of Yeshiva University
Feldman Marvin
Feldman Stephen E.
Kepplinger Esther M.
Zelson Steve T.
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