Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1996-10-11
1999-05-18
Saunders, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
436514, 436523, 436529, 436533, 436534, 436541, 436805, 436824, 436828, G01N33/537;33/546;33/548;33/558
Patent
active
059050284
ABSTRACT:
The present invention is a method useful for the detection of bloodgroup antigens and antibodies. The method of the present invention is envisioned for two types of assays: a direct assay and an indirect assay. The direct assay comprises adding a sample of erythrocytes to a reaction tube charged with two layers of immunoreactive particles, a first layer, preferably Sepharose, having Protein G coupled to the surface of those particles and a second layer, preferably Sephacryl S-200, having Protein A coupled to those particles in a buffer solution. Antibodies specific for the bloodgroup antigens tested for are coupled to the Protein G. The reaction tube is then centrifuged for a time sufficient to force to the bottom of the reaction tube erythrocytes that do not attach to the antibodies. The indirect assay comprises obtaining samples of erythrocytes, blood serum or blood plasma to be tested and mixing the erythrocytes, serum, or plasma with a known antibody or antigen reagent, depending on whether antigens or antibodies are being tested for. The mixture is incubated in a reaction tube above two layers of immunoreactive particles, a first layer, preferably Sepharose, having Protein G coupled to the surface of those particles and a second layer, preferably Sephacryl S-200, having Protein A coupled to those particles in a buffer solution. The reaction tube is centrifuged for a time sufficient to force to the bottom of the reaction tube erythrocytes that do not attach to the Protein G.
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Frame Thomas H.
Hatcher David E.
Moulds John J.
Gamma Biologicals, Inc.
Saunders David
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