Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1994-05-17
1997-09-09
Saunders, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
422 58, 422 59, 422 61, 422 681, 422 72, 422 73, 422 99, 422102, 4352872, 435975, 436501, 436514, 436518, 436528, 436529, 436533, 436534, 436541, 436805, 436808, 436809, 436810, 436824, 436828, G01N 33538, G01N 33546, G01N 33558, G01N 33566
Patent
active
056655584
ABSTRACT:
The present invention is a method and apparatus useful for the detection of bloodgroup antigens and antibodies. There are two preferred embodiments of the method: a direct assay and an indirect assay. The direct assay comprises adding a sample of erythrocytes to a reaction tube charged with a column of immunoreactive particles having an immunoglobulin binding ligand selected from the group consisting of Protein A, Protein G, Protein A/G or a universal kappa light chain binding protein coupled to the surface of the particles. Antibodies specific for bloodgroup antigens tested for are coupled to the ligand on the particles. The reaction tube is then centrifuged for a time sufficient to force to the bottom of the reaction tube erythrocytes that do not attach to the antibodies on the particles. The indirect assay comprises obtaining either a sample of erythrocytes or a sample of blood serum to be tested and mixing the erythrocytes or serum with a known antibody or antigen reagent, depending on whether antigens or antibodies are being tested for. The mixture is incubated in a reaction tube above a column of immunoreactive particles having immunoglobulin binding ligands selected from the group consisting of Protein A, Protein G, Protein A/G or a universal kappa light chain binding protein coupled to the surface of the particles. The reaction tube is centrifuged for a time sufficient to force to the bottom of the reaction tube erythrocytes that do not attach to the ligands on the particles. The response is the same regardless of whether a direct or indirect assay is performed.
REFERENCES:
patent: 3492396 (1970-01-01), Dalton et al.
patent: 3905772 (1975-09-01), Hartnett et al.
patent: 4105415 (1978-08-01), Lovett
patent: 4108972 (1978-08-01), Dreyer
patent: 4140662 (1979-02-01), Reckel et al.
patent: 4244694 (1981-01-01), Farina et al.
patent: 4391780 (1983-07-01), Boris
patent: 4433059 (1984-02-01), Chang et al.
patent: 4435293 (1984-03-01), Graham, Jr.
patent: 4436631 (1984-03-01), Graham et al.
patent: 4472357 (1984-09-01), Levy et al.
patent: 4478914 (1984-10-01), Giese
patent: 4486315 (1984-12-01), Skillman
patent: 4608231 (1986-08-01), Witty et al.
patent: 4659658 (1987-04-01), McCarthy et al.
patent: 4677080 (1987-06-01), Finkelstein
patent: 4698311 (1987-10-01), Hall et al.
patent: 4713198 (1987-12-01), Simonetti
patent: 4713348 (1987-12-01), Ullman
patent: 4829011 (1989-05-01), Gibbons
patent: 4894347 (1990-01-01), Hillyard et al.
patent: 5055259 (1991-10-01), Inoue et al.
patent: 5073341 (1991-12-01), Hargreaves
patent: 5086002 (1992-02-01), Hillyard et al.
patent: 5213963 (1993-05-01), Uthemann
patent: 5270167 (1993-12-01), Francoeur
patent: 5491067 (1996-02-01), Setcavage et al.
Wikstrom et al., "Proton Nuclear Magnetic Resonance Sequential Assignments and Secondary Structure of an Immunoglobulin Light Chain-Binding Domain of Protein L," Biochemistry 32:3381-3386 (1993).
Nilson et al., "Protein L from Peptostreptococcus magnus Binds to the K Light Chain Variable Domain," J. Biol. Chem. 267:2234-2238 (1992).
Kastern et al., "Protein L, a Bacterial Immunoglobulin-Binding Protein and Possible Virulence Determinant," Infect. and Immun. 58:1217-1222 (1990).
Bjorck et al., "Protein L: A Peptococcal Cell Wall Protein with Affinity for Immunoglobulin Light Chains," Bacterial Immunoglobulin-Binding Proteins 1:267-278 (1990).
Kastern et al., "Structure of Peptostreptococcal Protein L and Identification of a Repeated Immunoglobulin Light Chain-Binding Domain," J. Biol. Chem. 267:12820-12825 (1992).
Nilson et al., "Purification of Antibodies Using Protein L-Binding Framework Structures in the Light Chain Variable Domain," J. Immunol. Methods 164:33-40 (1993).
Chateau et al., "On the Interaction Between Protein L and Immunoglobins of Various Mammalian Species," Scand. J. Immunol. 37:399-405 (1993).
Patella et al., "Protein L: A Bacterial Ig-Binding Protein that Activates Human Basophils and Mast Cells,"0 J. Immunol. 145:3054-3061 (1990).
Lammler unler et al., "Characterization of Albumin-Binding Properties of Peptostreptococcus Magnus," Can. J. Microbiol. 35:614-618 (1989).
.ANG.kerstrom et al., "Protein L: An Immunoglobulin Light Chain-Binding Protein Characterization of Binding and Physicochemical Properties," J. Biol. Chem. 264:19740-19746 (1989).
Bjorck, "Protein L: A Novel Bacterial Cell Wall Protein with Affinity for Ig L Chains," J. Immunol. 140:1194-1197 (1988).
Myhre et al., "A Non-Immune Interaction Between the Light Chain of Human Immunoglobulin and a Surface Component of a Peptococcus Magnus Strain," Mol Immunol. 22:879-885 (1985).
Lapierre et al., "The gel test: a new way to detect red cell antigen-antibody reactions," Transfusion 30:109-113 (1990).
Pierce Catalog & Handbook, Life Science & Analytical Research Products, "Antibody/Protein Purification," pp. T59-T66 (1994).
Lewis et al., "Blood Group Terminology 1990," International Society of Blood Transfusion 58:152-159 (1990).
Dialog Search Report.
Anderson, "Analytical Technigues for Cell Fractions," Analytical Biochemistry 38:175-189 (1970).
Crowe, A.J., "Agglutination," Immunodiffusion, pp. 35-36 (1973).
Hitzler et al., "Gel Centrifugation Test--a New Micro Method for Blood Group Typing and Antibody Screening," Arztl. Lab. 35:89-92 (1989).
Basic & Clinical Immunology 3rd Edition, pp. 373-404 (1980).
Lisowski et al., "Sephadex Gel Filtration of Blood Cells," Archivum Immunologiae Et Therapiae Experimentals, pp. 387-390 (1966).
van Oss et al., "Influence of Various Physiochemical Factors on Hemagglutination," Vox Sang. 34:351-359 (1978).
American Association of Blood Banks Technical Manual 10th Edition, p. 279 (1990).
Lehninger, "The Molecular Basis of Cell Structure and Function," Biochemistry, pp. 141-143 (1970).
Frame Thomas H.
Hatcher David E.
Moulds John J.
Gamma Biologicals, Inc.
Saunders David
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