Method and apparatus to grow and mechanically condition cell...

Details Method and apparatus to grow and mechanically condition cell... Method and apparatus to grow and mechanically condition cell...

2006-02-14

2006-02-14

Beisner, William H. (Department: 1744)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Solid support and method of culturing cells on said solid...

C435S395000, C435S305100, C435S305200, C623S915000

Reexamination Certificate

active

06998265

ABSTRACT:
An apparatus (10) to grow cell cultures includes an anchor (14) potted to a flexible membrane (12) and an anchor stem (16) to which cells attach. A jig (18) having a trough (46) and at least one passage (52) is positioned adjacent the flexable membrane (12). A method for growing and mechanically conditioning three-dimensional cell constructs begins by drawing the flexible membrane (12) and the anchor stem (14) into the trough (46) by means (20) for flexing the flexible membrane (12) (step114). Cells are supplied (step116) and allowed to attach to the anchor stem (16) (step118). The flexible membrane (12) is released from within the trough (46) (step120), resulting in a three-dimensional structure (34) of cells attached to the anchor stem (16). The cells grow into the three-dimensional construct (step130). The structure (34) and/or the construct can be subjected to a regimen of strain for mechanical conditioning of the cells (step132).

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